Plasmid

Part:BBa_K1453901:Design

Designed by: Binhan Hao   Group: iGEM14_SJTU-BioX-Shanghai   (2014-10-01)


pBluescript II KS(+) EcoRV deletion


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 677
    Illegal SpeI site found at 683
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 683
    Illegal NotI site found at 669
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 689
    Illegal XhoI site found at 716
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 677
    Illegal SpeI site found at 683
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 677
    Illegal SpeI site found at 683
    Illegal NgoMIV site found at 328
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2089
    Illegal SapI site found at 1006


Design Notes

ScaI deletion


Source

pBluescript II KS(+)

References